​​​​​​
​​The Histone Source
​                                   Your source for histones, chromatin, and custom proteins
The Protein Expression and Purification (PEP) Facility

Customers' publications 
White AE, Hieb AR, and Luger K. A quantitative investigation of linker histone interactions with nucleosomes and chromatin. Sci Rep. 2016; 6: 19122.

Chen X, D'Arcy S, Radebaugh, CA,  Krzizike DD,  Giebler HA, Huang L,Nyborg JK, Luger K and Stargell SA.
Histone Chaperone Nap1 Is a Major Regulator of Histone H2A-H2B Dynamics at the Inducible GAL Locus.
Mol Cell Biol. 2016 Apr 15; 36(8): 1287–1296. 


Gansen A, Tóth K, Schwarz N, Langowski J. Opposing roles of H3- and H4-acetylation in the regulation of nucleosome structure––a FRET study.  Nucleic Acids Res. 2015 Feb 18;43(3):1433-43.

Zhang Q, Giebler HA, Isaacson  MK and Nyborg JK. Eviction of linker histone H1 by NAP-family histone chaperones enhances activated transcription. Epigenetics Chromatin. 2015; 8: 30.
       
Dechassa ML, Wyns K, and Luger K.  Scm3 deposits a (Cse4–H4)2 tetramer onto DNA through a Cse4–H4 dimer intermediate. Nucleic Acids Res. 2014 May 1; 42(9): 5532–5542.

Muthurajan UM, Hepler MR, Hieb, AR, Clark, NJ, Kramer M, Yao T and Luger K.  Automodification switches PARP-1 function from chromatin architectural protein to histone chaperone. Proc Natl Acad Sci U S A, 2014; 111; 12752-12757.  
 
D’Arcy S, Martin KW, Panchenko T, Chen X, Bergeron S, Stargell LA, Black BE and Luger K. Chaperone Nap1 shields histone surfaces used in a nucleosome and can put H2A-H2B in an unconventional tetrameric form. Mol Cell. 2013 Sep 12; 51(5): 662-77.
         
Clark NJ, Kramer M, Muthurajan UM, Luger K. Alternative Modes of Binding of Poly(ADP-ribose) Polymerase 1 to Free DNA and Nucleosomes. J Biol Chem. 2012; 287(39):32430-9.

Winkler DD, Zhou H, Dar MA, Zhang Z and Luger K. Yeast CAF-1 assembles histone (H3-H4)2 tetramers prior to DNA deposition. Nucleic Acids Res. 2012; Nov 1;40(20):10139-49.

Hieb, A.R., D’Arcy, S., Kramer, M.A., White, A.E. and Luger K. Fluorescence strategies for high-throughput quantification of protein interactions. Nucleic Acids Res. 2012; Mar;40(5):e33

Winkler DD, Muthurajan UM, Hieb AR and Luger K. Histone Chaperone FACT Coordinates Nucleosome Interaction through Multiple Synergistic Binding Events. J Biol Chem. 2011; Dec 2;286(48):41883-92.